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{"@context":"https://schema.org","@type":"DiscussionForumPosting","headline":"ERROR:\tmax count before zero is less than min required count (4) duplicates removed","articleBody":"Hi,\r\n\r\nEven if I found multiple times the error, I can't figure out what's wrong. I got this bam mapped with RNASeq pipeline from Nextflow, and I want to get curve (from SMARTSeq in fact). However, I have this error every time whatever I try.\r\n\r\n\r\n\r\n```\r\n$preseq lc_extrap -v -o out.nomerge.curve Exp16-12x-FS10-1-A02.sorted.bam\r\n\r\nBED_INPUT\r\nTOTAL READS     = 137084\r\nDISTINCT READS  = 137083\r\nDISTINCT COUNTS = 2\r\nMAX COUNT       = 2\r\nCOUNTS OF 1     = 137082\r\nMAX TERMS       = 2\r\nOBSERVED COUNTS (3)\r\n1\t137082\r\n2\t1\r\n\r\nERROR:\tmax count before zero is less than min required count (4) duplicates removed\r\n```\r\n\r\n```\r\n$preseq lc_extrap -v -o out.nomerge.curve -P -B Exp16-12x-FS10-1-A02.sorted.bam\r\n\r\nPAIRED_END_BED_INPUT\r\nERROR:\tproblem opening file: -B\r\nroot@zddm2-NV-PC:/home/zddm2/Documents# preseq lc_extrap -v -o out.nomerge.curve -B Exp16-12x-FS10-1-A02.sorted.bam\r\nBED_INPUT\r\nERROR:\tproblem opening file: -B\r\n```\r\n\r\nHere are my two lasts attempts, it seems there is a problem with the -B parameter? I tried with or without, I have one of the two error message.\r\nI installed preseq with  ./configure --enable-hts just to say,\r\n\r\nwould you have an idea? Thanks for your time!","author":{"url":"https://github.com/colin893","@type":"Person","name":"colin893"},"datePublished":"2024-03-27T20:46:07.000Z","interactionStatistic":{"@type":"InteractionCounter","interactionType":"https://schema.org/CommentAction","userInteractionCount":3},"url":"https://github.com/71/preseq/issues/71"}