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{"@context":"https://schema.org","@type":"DiscussionForumPosting","headline":"ERROR:  max count before zero is less than min required count (4) duplicates removed","articleBody":"Dear author\r\n\r\n\r\nThere are some problems in calculating library complexity using **preseq lc_extrap**. Versions over 3.0 do not have -B parameters. Using this version（preseq lc_extrap -e 10000  -P   -D -v -o   A1.test.dat SRR13810470-f2.q30.bam 2\u003eA1.test.log） will encounter an error (ERROR:  max count before zero is less than min required count (4) duplicates removed) in A1.test.log file and without generating A1.test.dat file. However, Versions below 2.0  have -B parameters. Using this version（preseq lc_extrap -e 10000  -P -B  -D -v -o   A1.test.dat SRR13810470-f2.q30.bam 2\u003eA1.test.log） will  generate two files without any errors. \r\n\r\nI want to know why  versions over 3.0 have this error.\r\n\r\nError as following\r\nPAIRED_END_BED_INPUT\r\nTOTAL READS     = 3213007\r\nDISTINCT READS  = 3.21293e+06\r\nDISTINCT COUNTS = 3\r\nMAX COUNT       = 3\r\nCOUNTS OF 1     = 3.21286e+06\r\nMAX TERMS       = 2\r\nOBSERVED COUNTS (4)\r\n1       3212858\r\n2       73\r\n3       1\r\n\r\nERROR:  max count before zero is less than min required count (4) duplicates removed\r\n","author":{"url":"https://github.com/liangwx332","@type":"Person","name":"liangwx332"},"datePublished":"2023-11-19T03:13:43.000Z","interactionStatistic":{"@type":"InteractionCounter","interactionType":"https://schema.org/CommentAction","userInteractionCount":0},"url":"https://github.com/68/preseq/issues/68"}