René's URL Explorer Experiment

{"@context":"https://schema.org","@type":"DiscussionForumPosting","headline":"Project 8: Explore the use of 10x Genomics' Linked-Reads to unlock currently inaccessible parts of the genome","articleBody":"**Project:**\n10x Genomics (http://software.10xgenomics.com/) has developed a technology that produces new type of long-range sequencing data called Linked-Reads. Long (\u003e100Kb) double stranded DNA is separated into millions of droplets, from which barcoded short-read libraries are produced. This produces sequencing libraries that are sequence-able on standard short-read sequencers, but which provide long-range information that is completely lost in standard short-read libraries. These barcoded short-reads, which can be traced back to their original long starting DNA molecules, are called Linked-Reads. Linked-Reads enable applications impossible with short-reads including diploid rather than haploid, resolution of mutation calls (i.e. phasing), high quality structural variation calling, true diploid de novo assembly, and the unlocking of parts of the genome that cannot be accurately assayed with short-read technologies. For this proposed project, we will explore use of Linked-Reads to unlock biologically and clinically important variants parts of the genome for which existing (i.e. pre-Linked-Read) technologies have been totally inadequate. The project will require developing novel algorithms and building upon algorithms that have already been built by 10x Genomicsäó» and customers to take full advantage of the long-range information contained in Linked-Reads.  \n\n**Project lead:** Michael Schnall-Levin / @mike10x / Industry Professional / 10x Genomics\n","author":{"url":"https://github.com/ttimbers","@type":"Person","name":"ttimbers"},"datePublished":"2016-06-13T15:02:32.000Z","interactionStatistic":{"@type":"InteractionCounter","interactionType":"https://schema.org/CommentAction","userInteractionCount":6},"url":"https://github.com/3/hackseq_projects_2016/issues/3"}